Cortical Spreading Depression is Associated with Arachidonic Acid Accumulation and Preservation of Energy Charge

M. Lauritzen*, A. J. Hansen, Dorte Kronborg, T. Wieloch

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The present study aimed to study the relation between the release of arachidonic acid (AA) and the energy state in cerebral cortices of rats during single episodes of cortical spreading depression (CSD). The changes in concentrations of AA, labile phosphate compounds [ATP, ADP, AMP, and phosphocreatine (PCr)], and glycolytic metabolites (lactate, pyruvate, glucose, and glycogen) were studied during and following the large change of the local direct current (DC) potential. Free AA increased markedly during the DC shift, continued to increase during the subsequent 3 min, and returned to control levels at 4-5 min after CSD. PCr decreased by 38% in the first minutes following the DC shift, while ADP increased by 38%. Both returned to normal within a few minutes. ATP, AMP, and energy charge remained constant throughout the experimental period. Glucose decreased by 47% and glycogen by 34% for a few minutes following CSD, while lactate increased by 105% at 2-3 min and by 77% at 4-5 min after CSD. The metabolites returned to control levels at 10 min after CSD. Considering the constant energy charge at all time points during CSD, it is suggested that the AA rise reflects augmented phospholipase activity due to either increased intracellular [Ca2+] or receptor stimulation or both. The possibility that N-methyl-D-aspartate receptors play a role in the release of AA, and that free AA in turn could be part of the mechanism of CSD, is discussed.

Original languageEnglish
JournalJournal of Cerebral Blood Flow and Metabolism
Volume10
Issue number1
Pages (from-to)115-122
Number of pages8
ISSN0271-678X
DOIs
Publication statusPublished - 1 Jan 1990
Externally publishedYes

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