Chemotaxis in Tetrahymena

Per Hellung-Larsen*, Vagn Leick, Niels Tommerup, Dorte Kronborg

*Kontaktforfatter af dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Chemotaxis — that is oriented locomotion of single cells — was shown by motion analysis of Tetrahymena thermophila. An electronic registration of swimming tracks was carried out in gradients of chemoattractant established in a modified Zigmond chamber. The attractants used were proteose peptone, platelet extract and fibroblast growth factor.
From 5 to 55 minutes after addition of the chemoattractant, 65% of the cells are oriented within the 180° angle segment towards the attractant compared to 51% in the control, containing no attractant. The figures are based on the measurement of 1567 and 499 tracks, respectively. Under the conditions used, cell migration towards the attractant is caused, solely, by an oriented movement (Chemotaxis) since the swimming speed was unaffected by the presence of the attractant. Similar results were obtained using T. pyriformis cells.

OriginalsprogEngelsk
TidsskriftEuropean Journal of Protistology
Vol/bind25
Udgave nummer3
Sider (fra-til)229-233
Antal sider5
ISSN0932-4739
DOI
StatusUdgivet - 1990
Udgivet eksterntJa

Citer dette

Hellung-Larsen, Per ; Leick, Vagn ; Tommerup, Niels ; Kronborg, Dorte. / Chemotaxis in Tetrahymena. I: European Journal of Protistology. 1990 ; Bind 25, Nr. 3. s. 229-233.
@article{5906304d414d4cb686053435c380a638,
title = "Chemotaxis in Tetrahymena",
abstract = "Chemotaxis — that is oriented locomotion of single cells — was shown by motion analysis of Tetrahymena thermophila. An electronic registration of swimming tracks was carried out in gradients of chemoattractant established in a modified Zigmond chamber. The attractants used were proteose peptone, platelet extract and fibroblast growth factor.From 5 to 55 minutes after addition of the chemoattractant, 65{\%} of the cells are oriented within the 180° angle segment towards the attractant compared to 51{\%} in the control, containing no attractant. The figures are based on the measurement of 1567 and 499 tracks, respectively. Under the conditions used, cell migration towards the attractant is caused, solely, by an oriented movement (Chemotaxis) since the swimming speed was unaffected by the presence of the attractant. Similar results were obtained using T. pyriformis cells.",
author = "Per Hellung-Larsen and Vagn Leick and Niels Tommerup and Dorte Kronborg",
year = "1990",
doi = "10.1016/s0932-4739(11)80174-4",
language = "English",
volume = "25",
pages = "229--233",
journal = "European Journal of Protistology",
issn = "0932-4739",
publisher = "Elsevier",
number = "3",

}

Hellung-Larsen, P, Leick, V, Tommerup, N & Kronborg, D 1990, 'Chemotaxis in Tetrahymena', European Journal of Protistology, bind 25, nr. 3, s. 229-233. https://doi.org/10.1016/s0932-4739(11)80174-4

Chemotaxis in Tetrahymena. / Hellung-Larsen, Per; Leick, Vagn; Tommerup, Niels; Kronborg, Dorte.

I: European Journal of Protistology, Bind 25, Nr. 3, 1990, s. 229-233.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Chemotaxis in Tetrahymena

AU - Hellung-Larsen, Per

AU - Leick, Vagn

AU - Tommerup, Niels

AU - Kronborg, Dorte

PY - 1990

Y1 - 1990

N2 - Chemotaxis — that is oriented locomotion of single cells — was shown by motion analysis of Tetrahymena thermophila. An electronic registration of swimming tracks was carried out in gradients of chemoattractant established in a modified Zigmond chamber. The attractants used were proteose peptone, platelet extract and fibroblast growth factor.From 5 to 55 minutes after addition of the chemoattractant, 65% of the cells are oriented within the 180° angle segment towards the attractant compared to 51% in the control, containing no attractant. The figures are based on the measurement of 1567 and 499 tracks, respectively. Under the conditions used, cell migration towards the attractant is caused, solely, by an oriented movement (Chemotaxis) since the swimming speed was unaffected by the presence of the attractant. Similar results were obtained using T. pyriformis cells.

AB - Chemotaxis — that is oriented locomotion of single cells — was shown by motion analysis of Tetrahymena thermophila. An electronic registration of swimming tracks was carried out in gradients of chemoattractant established in a modified Zigmond chamber. The attractants used were proteose peptone, platelet extract and fibroblast growth factor.From 5 to 55 minutes after addition of the chemoattractant, 65% of the cells are oriented within the 180° angle segment towards the attractant compared to 51% in the control, containing no attractant. The figures are based on the measurement of 1567 and 499 tracks, respectively. Under the conditions used, cell migration towards the attractant is caused, solely, by an oriented movement (Chemotaxis) since the swimming speed was unaffected by the presence of the attractant. Similar results were obtained using T. pyriformis cells.

UR - https://sfx-45cbs.hosted.exlibrisgroup.com/45cbs?url_ver=Z39.88-2004&url_ctx_fmt=info:ofi/fmt:kev:mtx:ctx&ctx_enc=info:ofi/enc:UTF-8&ctx_ver=Z39.88-2004&rfr_id=info:sid/sfxit.com:azlist&sfx.ignore_date_threshold=1&rft.object_id=954925569824&rft.object_portfolio_id=&svc.holdings=yes&svc.fulltext=yes

U2 - 10.1016/s0932-4739(11)80174-4

DO - 10.1016/s0932-4739(11)80174-4

M3 - Journal article

VL - 25

SP - 229

EP - 233

JO - European Journal of Protistology

JF - European Journal of Protistology

SN - 0932-4739

IS - 3

ER -